The effect of type II toxin-antitoxin systems on methicillinresistant Staphylococcus aureus persister cell formation and antibiotic tolerance
نویسندگان
چکیده
Persister cells are defi ned as a subpopulation of bacteria in dormant state with the ability to reduce bacterial metabolism and they involved antibiotic tolerance. Toxin-antitoxin (TA) systems have been previously suggested important players persistence. Therefore, this study aimed involvement TA persister cell formation methicillin-resistant Staphylococcus aureus following exposure. Using TADB RASTA database, two type II including MazF/MazE RelE/RelB were predicted S. aureus. The presence these genes was determined 5 isolates standard strain subsp. N315 using PCR method. To induce persistence, exposed lethal doses ciprofl oxacin expression studied system measured after h Real-Time PCR. According our results, all harbored genes. highly capable exposure sub-MIC RT-qPCR showed signifi cant increase MazEF RelBE loci, indicating their potential role Considering importance tolerance, further studies on phenomenon required effi ciently target systems.
منابع مشابه
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ژورنال
عنوان ژورنال: Acta biologica Szegediensis /
سال: 2021
ISSN: ['1588-385X', '1588-4082']
DOI: https://doi.org/10.14232/abs.2021.1.113-117